C6904

Sigma

 

Concanavalin A from Canavalia ensiformis (Jack bean)

agarose conjugate, Type V-A, buffered aqueous suspension

Synonym:Con A
CAS Number:11028-71-0
MDL number:MFCD00071069

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Description

Analysis NoteWhere reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Biochem/physiol ActionsCon A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Physical formSuspension in 0.1 M acetate buffer, pH 6.0, containing 1 M NaCl, 1 mM Mn2+, 1 mM Ca2+, 1 mM Mg2+ and 0.01% thimerosal

Properties

typeType V-A
formbuffered aqueous suspension
extent of labeling~10 mg per mL
matrix 4% beaded agarose
matrix activationp-nitrophenyl chloroformate
matrix spacer1 atom
capacity 3-6 mg/mL binding capacity (yeast mannan)
conjugateagarose conjugate
storage temp.2-8°C

Safety

Hazard CodesXn
Risk Statements42/43
Safety Statements23-36/37-45
WGK Germany3

References

referenceReeke, G.N., et al. Ann. N.Y. Acad. Sci. 234, 369, (1974)
 Kalb, A.J. and Lustig, A. Biochim. Biophys. Acta 168, 366, (1968)
 Gunther, G.R., et al. Proc. Natl. Acad. Sci. U. S. A. 70, 1012, (1973)
 , Bittiges, H. and Schnebli, H.P, ed. Concanavalin A as a Tool London , (1976)
MerckMerck 13,2521